PT  - JOURNAL ARTICLE
AU  - Pei Tong
AU  - Avneesh Gautam
AU  - Ian Windsor
AU  - Meghan Travers
AU  - Yuezhou Chen
AU  - Nicholas Garcia
AU  - Noah B. Whiteman
AU  - Lindsay G.A. McKay
AU  - Felipe J.N. Lelis
AU  - Shaghayegh Habibi
AU  - Yongfei Cai
AU  - Linda J. Rennick
AU  - W. Paul Duprex
AU  - Kevin R. McCarthy
AU  - Christy L. Lavine
AU  - Teng Zuo
AU  - Junrui Lin
AU  - Adam Zuiani
AU  - Jared Feldman
AU  - Elizabeth A. MacDonald
AU  - Blake M. Hauser
AU  - Anthony Griffths
AU  - Michael S. Seaman
AU  - Aaron G. Schmidt
AU  - Bing Chen
AU  - Donna Neuberg
AU  - Goran Bajic
AU  - Stephen C. Harrison
AU  - Duane R. Wesemann
TI  - Memory B cell repertoire for recognition of evolving SARS-CoV-2 spike
AID  - 10.1101/2021.03.10.434840
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.03.10.434840
4099  - http://biorxiv.org/content/early/2021/03/10/2021.03.10.434840.short
4100  - http://biorxiv.org/content/early/2021/03/10/2021.03.10.434840.full
AB  - Memory B cell reserves can generate protective antibodies against repeated SARS-CoV-2 infections, but with an unknown reach from original infection to antigenically drifted variants. We charted memory B cell receptor-encoded monoclonal antibodies (mAbs) from 19 COVID-19 convalescent subjects against SARS-CoV-2 spike (S) and found 7 major mAb competition groups against epitopes recurrently targeted across individuals. Inclusion of published and newly determined structures of mAb-S complexes identified corresponding epitopic regions. Group assignment correlated with cross-CoV-reactivity breadth, neutralization potency, and convergent antibody signatures. mAbs that competed for binding the original S isolate bound differentially to S variants, suggesting the protective importance of otherwise-redundant recognition. The results furnish a global atlas of the S-specific memory B cell repertoire and illustrate properties conferring robustness against emerging SARS-CoV-2 variants.Competing Interest StatementThe authors have declared no competing interest.