RT Journal Article
SR Electronic
T1 No cell is an island: circulating T cell:monocyte complexes are markers of immune perturbations
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 411553
DO 10.1101/411553
A1 Julie G. Burel
A1 Mikhail Pomaznoy
A1 Cecilia S. Lindestam Arlehamn
A1 Daniela Weiskopf
A1 Ricardo da Silva Antunes
A1 Yunmin Jung
A1 Mariana Babor
A1 Veronique Schulten
A1 Grégory Seumois
A1 Jason A. Greenbaum
A1 Sunil Premawansa
A1 Gayani Premawansa
A1 Ananda Wijewickrama
A1 Dhammika Vidanagama
A1 Bandu Gunasena
A1 Rashmi Tippalagama
A1 Aruna D. deSilva
A1 Robert H. Gilman
A1 Mayuko Saito
A1 Randy Taplitz
A1 Klaus Ley
A1 Pandurangan Vijayanand
A1 Alessandro Sette
A1 Bjoern Peters
YR 2019
UL http://biorxiv.org/content/early/2019/02/21/411553.abstract
AB Our results highlight for the first time that a significant proportion of cell doublets in flow cytometry, previously believed to be the result of technical artefacts and thus ignored in data acquisition and analysis, are the result of true biological interaction between immune cells. In particular, we show that cell:cell doublets pairing a T cell and a monocyte can be directly isolated from human blood, and high resolution microscopy shows polarized distribution of LFA1/ICAM1 in many doublets, suggesting in vivo formation. Intriguingly, T cell:monocyte complex frequency and phenotype fluctuate with the onset of immune perturbations such as infection or immunization, reflecting expected polarization of immune responses. Overall these data suggest that cell doublets reflecting T cell-monocyte in vivo immune interactions can be detected in human blood and that the common approach in flow cytometry to avoid studying cell:cell complexes should be revisited.