RT Journal Article
SR Electronic
T1 Endosomal Membrane Tension Controls ESCRT-III-Dependent Intra-Lumenal Vesicle Formation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 550483
DO 10.1101/550483
A1 Mercier, Vincent
A1 Larios, Jorge
A1 Molinard, Guillaume
A1 Goujon, Antoine
A1 Matile, Stefan
A1 Gruenberg, Jean
A1 Roux, Aurélien
YR 2019
UL http://biorxiv.org/content/early/2019/02/21/550483.abstract
AB Plasma membrane tension strongly affects cell surface processes, such as migration, endocytosis and signalling. However, it is not known whether membrane tension of organelles regulates their functions, notably intracellular traffic. The ESCRT-III complex is the major membrane remodelling complex that drives Intra-Lumenal Vesicle (ILV) formation on endosomal membranes. Here, we made use of a new fluorescent membrane tension probe to show that ESCRT-III subunits are recruited onto endosomal membranes when membrane tension is reduced. We find that tension-dependent recruitment is associated with ESCRT-III polymerization and membrane deformation in vitro, and correlates with increased ILVs formation in ESCRT-III decorated endosomes in vivo. Finally, we find that endosomal membrane tension decreases when ILV formation is triggered by EGF under physiological conditions. These results indicate that membrane tension is a major regulator of ILV formation and of endosome trafficking, leading us to conclude that membrane tension can control organelle functions.One Sentence Summary Membrane tension decrease facilitates membrane remodeling by ESCRT-III polymerization during intra-lumenal vesicle formation.