PT  - JOURNAL ARTICLE
AU  - Hélène Badouin
AU  - Marie-Claude Boniface
AU  - Nicolas Pouilly
AU  - Anne-Laure Fuchs
AU  - Felicity Vear
AU  - Nicolas B. Langlade
AU  - Jérôme Gouzy
AU  - Stéphane Muños
TI  - Pooled Single-Molecule transcriptomics identifies a giant gene under balancing selection in sunflower
AID  - 10.1101/2021.03.17.435796
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.03.17.435796
4099  - http://biorxiv.org/content/early/2021/03/19/2021.03.17.435796.short
4100  - http://biorxiv.org/content/early/2021/03/19/2021.03.17.435796.full
AB  - Genes under balancing selection control phenotypes such as immunity, color or sex, but are difficult to identify. Self-incompatibility genes are under negative frequency-dependent selection, a special case of balancing selection, with up to 30 to 50 alleles segregating per population. We developed a method based on pooled Single-Molecule transcriptomics to identify balanced polymorphisms expressed in tissues of interest. We searched for multi-allelic, non-recombining genes causing self-incompatibility in wild sunflower (Helianthus annuus). A diversity scan in pistil identified a gene, Ha7650b,that displayed balanced polymorphism and colocalized with a quantitative trait locus for self-incompatibility. Unexpectedly, Ha7650b displayed gigantism (400 kb), which was caused by increase in intron size as a consequence of suppressed recombination. Ha7650b emerged after a whole-genome duplication (29 millions years ago) followed by tandem duplications and neofunctionalisation. Ha7650b shows expression, genetic location, genomic neighbourhood and predicted function that provide strong evidence that it is involved in self-incompatibility. Pooled Single-Molecule transcriptomics is an affordable and powerful new method that makes it possible to identify diversity and structural outliers simultaneously. It will allow a breakthrough in the discovery of self-incompatibility genes and other expressed genes under balancing selection.Competing Interest StatementThe authors have declared no competing interest.