PT  - JOURNAL ARTICLE
AU  - Renato R. M. Oliveira
AU  - Raíssa Silva
AU  - Gisele L. Nunes
AU  - Guilherme Oliveira
TI  - PIMBA: a PIpeline for MetaBarcoding Analysis
AID  - 10.1101/2021.03.23.436646
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.03.23.436646
4099  - http://biorxiv.org/content/early/2021/03/23/2021.03.23.436646.short
4100  - http://biorxiv.org/content/early/2021/03/23/2021.03.23.436646.full
AB  - Summary DNA metabarcoding is an emerging monitoring method capable of assessing biodiversity from environmental samples (eDNA). Advances in computational tools have been required due to the increase of Next-Generation Sequencing data. Tools for DNA metabarcoding analysis, such as MOTHUR, QIIME, Obitools, and mBRAVE have been widely used in ecological studies. However, some difficulties are encountered when there is a need to use custom databases. Here we present PIMBA, a PIpeline for MetaBarcoding Analysis, which allows the use of customized databases, as well as other reference databases used by the softwares mentioned here. PIMBA is an open-source and user-friendly pipeline that consolidates all analyses in just three command lines.Availability and Implementation https://github.com/reinator/pimbaContact renato.renison{at}gmail.com and guilherme.oliveira{at}pq.itv.org.Competing Interest StatementThe authors have declared no competing interest.