PT  - JOURNAL ARTICLE
AU  - Reema Baskar
AU  - Amy F. Chen
AU  - Patricia Favaro
AU  - Warren Reynolds
AU  - Fabian Mueller
AU  - Luciene Borges
AU  - Sizun Jiang
AU  - Hyun Shin Park
AU  - Eric T. Kool
AU  - William J. Greenleaf
AU  - Sean C. Bendall
TI  - Integrating transcription factor abundance with chromatin accessibility in human erythroid lineage commitment
AID  - 10.1101/2021.03.23.436609
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.03.23.436609
4099  - http://biorxiv.org/content/early/2021/03/24/2021.03.23.436609.short
4100  - http://biorxiv.org/content/early/2021/03/24/2021.03.23.436609.full
AB  - We present InTAC-seq, a method for simultaneous quantification of genome-wide open chromatin and intracellular protein abundance in fixed cells. Using InTAC we directly observe variation in chromatin accessibility and transcription factor motif occupancy driven by differences in transcription factor protein abundance. By purifying bone marrow progenitor cells based on GATA1 protein expression, we establish its role in both functional and epigenetic restriction of erythroid cell identity in human hematopoiesis.Competing Interest StatementThe authors have declared no competing interest.