PT  - JOURNAL ARTICLE
AU  - Michael J. Wester
AU  - David J. Schodt
AU  - Hanieh Mazloom-Farsibaf
AU  - Mohamadreza Fazel
AU  - Sandeep Pallikkuth
AU  - Keith A. Lidke
TI  - Robust, Fiducial-Free Drift Correction for Super-resolution Imaging
AID  - 10.1101/2021.03.26.437196
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.03.26.437196
4099  - http://biorxiv.org/content/early/2021/03/27/2021.03.26.437196.short
4100  - http://biorxiv.org/content/early/2021/03/27/2021.03.26.437196.full
AB  - We describe a robust, fiducial-free method of drift correction for use in single molecule localization-based super-resolution methods. The method combines periodic 3D registration of the sample using brightfield images with a fast post-processing algorithm that corrects residual registration errors and drift between registration events. The method is robust to low numbers of collected localizations, requires no specialized hardware, and provides stability and drift correction for an indefinite time period.Competing Interest StatementThe authors have declared no competing interest.