PT  - JOURNAL ARTICLE
AU  - Valeria Mateo-Estrada
AU  - José Luis Fernández-Vázquez
AU  - Julia Moreno-Manjón
AU  - Ismael L. Hernández-González
AU  - Eduardo Rodríguez-Noriega
AU  - Rayo Morfín-Otero
AU  - María Dolores Alcántar-Curiel
AU  - Santiago Castillo-Ramírez
TI  - Accessory genomic epidemiology of co-circulating <em>Acinetobacter baumannii</em> clones
AID  - 10.1101/2021.03.26.436874
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.03.26.436874
4099  - http://biorxiv.org/content/early/2021/03/26/2021.03.26.436874.short
4100  - http://biorxiv.org/content/early/2021/03/26/2021.03.26.436874.full
AB  - A. baumannii has become one of the most important multidrug resistant nosocomial pathogens all over the world. Nonetheless, very little is known about the diversity of A. baumannii lineages co-existing in hospital settings. Here, using whole-genome sequencing, epidemiological data and antimicrobial susceptibility tests, we uncover the transmission dynamics of extensive and multidrug resistant A. baumannii in a tertiary hospital for a decade. Our core genome phylogeny of almost 300 genomes suggests that there were several introductions of lineages from international clone 2 into the hospital. The molecular dating analysis shows that these introductions happened between 2004 and 2015. Furthermore, using the accessory genome, we show that these lineages were extensively disseminated across many wards in the hospital. Our results demonstrate that accessory genome variation can be a very powerful tool for conducting genomic epidemiology. We anticipate future studies employing the accessory genome as a phylogenomic marker over very short microevolutionary scales.Competing Interest StatementThe authors have declared no competing interest.