RT Journal Article SR Electronic T1 Accessory genomic epidemiology of co-circulating Acinetobacter baumannii clones JF bioRxiv FD Cold Spring Harbor Laboratory SP 2021.03.26.436874 DO 10.1101/2021.03.26.436874 A1 Mateo-Estrada, Valeria A1 Fernández-Vázquez, José Luis A1 Moreno-Manjón, Julia A1 Hernández-González, Ismael L. A1 Rodríguez-Noriega, Eduardo A1 Morfín-Otero, Rayo A1 Alcántar-Curiel, María Dolores A1 Castillo-Ramírez, Santiago YR 2021 UL http://biorxiv.org/content/early/2021/03/26/2021.03.26.436874.abstract AB A. baumannii has become one of the most important multidrug resistant nosocomial pathogens all over the world. Nonetheless, very little is known about the diversity of A. baumannii lineages co-existing in hospital settings. Here, using whole-genome sequencing, epidemiological data and antimicrobial susceptibility tests, we uncover the transmission dynamics of extensive and multidrug resistant A. baumannii in a tertiary hospital for a decade. Our core genome phylogeny of almost 300 genomes suggests that there were several introductions of lineages from international clone 2 into the hospital. The molecular dating analysis shows that these introductions happened between 2004 and 2015. Furthermore, using the accessory genome, we show that these lineages were extensively disseminated across many wards in the hospital. Our results demonstrate that accessory genome variation can be a very powerful tool for conducting genomic epidemiology. We anticipate future studies employing the accessory genome as a phylogenomic marker over very short microevolutionary scales.Competing Interest StatementThe authors have declared no competing interest.