RT Journal Article
SR Electronic
T1 Generation and first characterization of TRDC-Knockout pigs lacking γδ T cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.03.27.437312
DO 10.1101/2021.03.27.437312
A1 Bjoern Petersen
A1 Robert Kammerer
A1 Antje Frenzel
A1 Petra Hassel
A1 Tung Huy Dau
A1 Roswitha Becker
A1 Angele Breithaupt
A1 Reiner Georg Ulrich
A1 Andrea Lucas-Hahn
A1 Gregor Meyers
YR 2021
UL http://biorxiv.org/content/early/2021/03/29/2021.03.27.437312.abstract
AB The TRDC-Locus encodes the T cell receptor delta constant region, one component of the γδ T cell receptor which is essential for development of γδ T cells. In contrast to peptide recognition by αβ T cells, antigens activating γδ T cells are mostly MHC independent and not well characterized. Therefore, the function of γδ T cells and their contribution to protection against infections is still unclear. Higher numbers of circulating γδ T cells compared to mice, render the pig a suitable animal model to study γδ T cells. Knocking-out the porcine TRDC-locus by intracytoplasmic microinjection and somatic cell nuclear transfer resulted in healthy living γδ T cell deficient offspring. Flow cytometric analysis revealed that TRDC-KO pigs lack γδ T cells in peripheral blood mononuclear cells (PBMC) and spleen cells. The composition of the remaining leucocyte subpopulations was not affected by the depletion of γδ T cells. Genome-wide transcriptome analyses in PBMC revealed a pattern of changes reflecting the impairment of known or expected γδ T cell dependent pathways. Histopathology did not reveal developmental abnormalities of secondary lymphoid tissues. However, in a vaccination experiment the KO pigs stayed healthy but had a significantly lower neutralizing antibody titer as the syngenic controls.Competing Interest StatementThe authors have declared no competing interest.