TY - JOUR T1 - Unconventional Translation Initiation Factor EIF2A is required for Drosophila spermatogenesis JF - bioRxiv DO - 10.1101/2021.03.28.437419 SP - 2021.03.28.437419 AU - David D. Lowe AU - Denise J. Montell Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/03/29/2021.03.28.437419.abstract N2 - The eukaryotic initiation factor EIF2A is an unconventional translation factor required for initiation of protein synthesis from non-AUG codons from a variety of transcripts, including oncogenes and stress related genes in mammalian cells. Its function in multicellular organisms has not been reported. Here, we identify and characterize mutant alleles of the CG7414 gene, which encodes the Drosophila EIF2A ortholog. We identified that CG7414 undergoes sex-specific splicing that regulates its male-specific expression. We characterized a Mi{Mic} transposon insertion that disrupts the coding regions of all predicted isoforms and is a genetic null allele, and a PBac transposon insertion into an intron, which is a hypomorph. The Mi{Mic} allele is homozygous lethal, while the viable progeny from the hypomorphic PiggyBac allele are male sterile and female fertile. In dEIF2A mutant flies, sperm failed to individualize due to defects in F-actin cones and failure to form and maintain cystic bulges, ultimately leading to sterility. These results demonstrate that EIF2A is essential in a multicellular organism, both for normal development and spermatogenesis, and provide an entree into the elucidation of the role of EIF2A and unconventional translation in vivo.Competing Interest StatementThe authors have declared no competing interest. ER -