RT Journal Article SR Electronic T1 Fast and accurate de novo genome assembly from long uncorrected reads JF bioRxiv FD Cold Spring Harbor Laboratory SP 068122 DO 10.1101/068122 A1 Vaser, Robert A1 Sović, Ivan A1 Nagarajan, Niranjan A1 Šikić, Mile YR 2016 UL http://biorxiv.org/content/early/2016/08/05/068122.abstract AB The assembly of long reads from Pacific Biosciences and Oxford Nanopore Technologies typically requires resource intensive error correction and consensus generation steps to obtain high quality assemblies. We show that the error correction step can be omitted and high quality consensus sequences can be generated efficiently with a SIMD accelerated, partial order alignment based stand-alone consensus module called Racon. Based on tests with PacBio and Oxford Nanopore datasets we show that Racon coupled with Miniasm enables consensus genomes with similar or better quality than state-of-the-art methods while being an order of magnitude faster.Racon is available open source under the MIT license at https://github.com/isovic/racon.git.