TY - JOUR T1 - Establishment and Resilience of Transplanted Gut Microbiota in Aged Mice JF - bioRxiv DO - 10.1101/2021.03.18.435923 SP - 2021.03.18.435923 AU - Ying Wang AU - Jinhui Tang AU - Qingqing Lv AU - Yuxiang Tan AU - Xiaoxiao Dong AU - Hongbin Liu AU - Nannan Zhao AU - Zhen He AU - Yan Kou AU - Yan Tan AU - Xin-an Liu AU - Liping Wang AU - Yang-Yu Liu AU - Lei Dai Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/03/30/2021.03.18.435923.abstract N2 - Fecal microbiota transplantation (FMT), a procedure in which fecal material is transferred from a donor to a recipient, has been increasingly used as a treatment to restore healthy gut microbiota. There is a substantial difference in the composition of gut microbiota between young and aged hosts, but little is known about whether age matching between the FMT donor and recipient affects microbiota restoration and long-term maintenance. In the present investigation, we aimed to study the establishment and resilience of transplanted gut microbiota in aged recipients. We treated naturally aged mice (20 months old) with a broad-spectrum antibiotic cocktail and monitored the restoration of gut microbiota over 8 weeks. The diversity of gut microbiota in aged mice failed to reach the baseline level via spontaneous recovery; in contrast, FMT from either (age-)matched or unmatched donors facilitated the recovery of gut microbiota diversity. The microbiota transplanted from different donors successfully established in the aged recipients and had long-term effects on the gene expression profiles of the host colon. Finally, we evaluated the long-term maintenance of transplanted microbiota via intentional disruption of gut homeostasis. We found that lack of age matching between FMT donors and recipients may decrease the resilience of transplanted gut microbiota against colonic inflammation. The results from our study systematically examining the effects of FMT on the gut homeostasis of aged hosts suggest that the compatibility between donors and recipients should be taken into account when implementing FMT.Competing Interest StatementThe authors have declared no competing interest. ER -