RT Journal Article
SR Electronic
T1 A Broadly Conserved Deoxycytidine Deaminase Protects Bacteria from Phage Infection
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.03.31.437871
DO 10.1101/2021.03.31.437871
A1 Geoffrey B. Severin
A1 Brian Y. Hsueh
A1 Clinton A. Elg
A1 John A. Dover
A1 Christopher R. Rhoades
A1 Alex J. Wessel
A1 Benjamin J. Ridenhour
A1 Eva M. Top
A1 Janani Ravi
A1 Kristin N. Parent
A1 Christopher M. Waters
YR 2021
UL http://biorxiv.org/content/early/2021/03/31/2021.03.31.437871.abstract
AB The El Tor biotype of Vibrio cholerae is responsible for perpetuating the longest cholera pandemic in recorded history (1961-current). The genomic islands VSP-1 and -2 are two understudied genetic features that distinguish El Tor from previous pandemics. To understand their utility, we calculated the co-occurrence of VSP genes across bacterial genomes. This analysis predicted the previously uncharacterized vc0175, herein renamed deoxycytidylate deaminase Vibrio (dcdV), is in a gene network with dncV, a cyclic GMP-AMP synthase involved in phage defense. DcdV consists of two domains, a P-loop kinase and a deoxycytidylate deaminase, that are required for the deamination of dCTP and dCMP, inhibiting phage predation by corrupting cellular nucleotide concentrations. Additionally, DcdV is post-translationally inhibited by a unique noncoding RNA encoded 5’ of the dcdV locus. DcdV homologs are conserved in bacteria and eukaryotes and our results identify V. cholerae DcdV as the founding member of a previously undescribed bacterial phage defense system.Competing Interest StatementThe authors have declared no competing interest.