PT  - JOURNAL ARTICLE
AU  - Prischich, Davia
AU  - del Dedo, Javier Encinar
AU  - Cambra, Maria
AU  - Prat, Judit
AU  - Camarero, Nuria
AU  - Nevola, Laura
AU  - Martín-Quirós, Andrés
AU  - Rebollo, Elena
AU  - Giralt, Ernest
AU  - Isabel Geli, María
AU  - Gorostiza, Pau
TI  - Light-dependent inhibition of clathrin-mediated endocytosis in yeast
AID  - 10.1101/2021.04.01.432428
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.04.01.432428
4099  - http://biorxiv.org/content/early/2021/04/01/2021.04.01.432428.short
4100  - http://biorxiv.org/content/early/2021/04/01/2021.04.01.432428.full
AB  - Clathrin-mediated endocytosis (CME) is an essential cellular process, which is evolutionarily conserved among eukaryotes. Yeast constitutes a powerful genetic model to dissect the complex endocytic machinery, yet there is a lack of pharmacological agents that could complement genetics in selectively and reversibly interfere with CME in these organisms. TL2 is a light-regulated peptide inhibitor that targets the AP2/β-arrestin interaction and that can photocontrol CME with high spatiotemporal precision in mammalian cells. Here, we study endocytic protein dynamics by live-cell imaging of the fluorescently tagged coat-associated protein Sla1-GFP and demonstrate that TL2 retains its inhibitory activity in S. cerevisiae spheroplasts, thus providing a unique tool for acute and reversible CME modulation in yeast.Competing Interest StatementThe authors have declared no competing interest.