RT Journal Article
SR Electronic
T1 Light-dependent inhibition of clathrin-mediated endocytosis in yeast
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.04.01.432428
DO 10.1101/2021.04.01.432428
A1 Prischich, Davia
A1 del Dedo, Javier Encinar
A1 Cambra, Maria
A1 Prat, Judit
A1 Camarero, Nuria
A1 Nevola, Laura
A1 Martín-Quirós, Andrés
A1 Rebollo, Elena
A1 Giralt, Ernest
A1 Isabel Geli, María
A1 Gorostiza, Pau
YR 2021
UL http://biorxiv.org/content/early/2021/04/01/2021.04.01.432428.abstract
AB Clathrin-mediated endocytosis (CME) is an essential cellular process, which is evolutionarily conserved among eukaryotes. Yeast constitutes a powerful genetic model to dissect the complex endocytic machinery, yet there is a lack of pharmacological agents that could complement genetics in selectively and reversibly interfere with CME in these organisms. TL2 is a light-regulated peptide inhibitor that targets the AP2/β-arrestin interaction and that can photocontrol CME with high spatiotemporal precision in mammalian cells. Here, we study endocytic protein dynamics by live-cell imaging of the fluorescently tagged coat-associated protein Sla1-GFP and demonstrate that TL2 retains its inhibitory activity in S. cerevisiae spheroplasts, thus providing a unique tool for acute and reversible CME modulation in yeast.Competing Interest StatementThe authors have declared no competing interest.