PT - JOURNAL ARTICLE AU - Harsh Pandey AU - Shivani Srivastava AU - Mohan Kumar AU - Yamini Bhusan Tripathi TI - Histopathological Study Of Different Organs Of Charles Foster Rats Under The Exposure Of <em>Pueraria tuberosa</em> AID - 10.1101/671529 DP - 2021 Jan 01 TA - bioRxiv PG - 671529 4099 - http://biorxiv.org/content/early/2021/04/04/671529.short 4100 - http://biorxiv.org/content/early/2021/04/04/671529.full AB - The present study was undertaken to investigate the safe doses of Pueraria tuberosa water extract (PTWE) on different organs. The OECD guidelines 407 of repeated toxicity was followed with respect to the selection of dose and days for different organs. The selected doses of PTWE were 250, 500, 1000 and 2000 mg/kg b wt for 7, 14, 21 and 28 days. Haematoxylin and eosin staining was used to study the morphological alterations in heart, intestine, testis, adrenal gland and spleen. In the present study, no adverse alterations in cardiac fibers of the heart, size and shapes in crypts and villi of intestine, seminiferous tubules and spermatozoa count in testis, three zones of adrenal gland, and spleen were seen in all treated groups of PTWE. There were no adverse morphological alterations found in described organs. The PTWE are safe at 1000 mg/kg b wt. up to 28 days and 2000 mg/ kg b. wt up to 21 days, respectively.Competing Interest StatementThe authors have declared no competing interest.