RT Journal Article
SR Electronic
T1 PRMT5 regulates ovarian follicle development by facilitating <em>Wt1</em> translation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.04.04.438394
DO 10.1101/2021.04.04.438394
A1 Min Chen
A1 Fangfang Dong
A1 Min Chen
A1 Zhiming Shen
A1 Haowei Wu
A1 Changhuo Cen
A1 Xiuhong Cui
A1 Shilai Bao
A1 Fei Gao
YR 2021
UL http://biorxiv.org/content/early/2021/04/04/2021.04.04.438394.abstract
AB Protein arginine methyltransferase 5 (Prmt5) is the major type II enzyme responsible for symmetric dimethylation of arginine. Here, we found PRMT5 was expressed at high level in ovarian granulosa cells of growing follicles. Inactivation of Prmt5 in granulosa cells resulted in aberrant follicle development and female infertility. In Prmt5-knockout mice, follicle development was arrested with disorganized granulosa cells in which WT1 expression was dramatically reduced and the expression of steroidogenesis-related genes was significantly increased. The premature differentiated granulosa cells were detached from oocytes and follicle structure was disrupted. Mechanism studies revealed that Wt1 expression was regulated by PRMT5 at the protein level. PRMT5 facilitated IRES-dependent translation of Wt1 mRNA by methylating HnRNPA1. Moreover, the upregulation of steroidogenic genes in Prmt5-deficient granulosa cells was repressed by Wt1 overexpression. These results demonstrate PRMT5 participates in granulosa cell lineage maintenance by inducing Wt1 expression. Our study uncovers a new role of post-translational arginine methylation in granulosa cell differentiation and follicle development.Competing Interest StatementThe authors have declared no competing interest.