RT Journal Article
SR Electronic
T1 Driving potent neutralization of a SARS-CoV-2 Variant of Concern with a heterotypic boost
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.04.03.438330
DO 10.1101/2021.04.03.438330
A1 Sheward, Daniel J.
A1 Mandolesi, Marco
A1 Kim, Changil
A1 Hanke, Leo
A1 Vidakovics, Laura Perez
A1 McInerney, Gerald
A1 Hedestam, Gunilla B. Karlsson
A1 Murrell, Ben
YR 2021
UL http://biorxiv.org/content/early/2021/04/05/2021.04.03.438330.abstract
AB The emergence of SARS-CoV-2 Variants of Concern (VOCs) with mutations in key neutralizing antibody epitopes threatens to undermine vaccines developed against the pandemic founder variant (Wu-Hu-1). Widespread vaccine rollout and continued transmission are creating a population that has antibody responses of varying potency to Wu-Hu-1. Against this background, it is critical to assess the outcomes of subsequent booster vaccination with variant antigens. It is not yet known whether such heterotypic vaccine boosts would be compromised by original antigenic sin, where pre-existing responses to a prior variant dampen responses to a new one, or whether the primed memory B cell repertoire would bridge the gap between Wu-Hu-1 and VOCs. Here, we show that a single adjuvanted dose of receptor binding domain (RBD) protein from VOC 501Y.V2 (B.1.351) drives an extremely potent neutralizing antibody response capable of cross-neutralizing both Wu-Hu-1 and 501Y.V2 in rhesus macaques previously immunized with Wu-Hu-1 spike protein.Competing Interest StatementThe authors have declared no competing interest.