TY - JOUR T1 - Computational Simulations of the 4-D Micro-Circulatory Network in Zebrafish Tail Amputation and Regeneration JF - bioRxiv DO - 10.1101/2021.02.10.430654 SP - 2021.02.10.430654 AU - Mehrdad Roustaei AU - Kyung In Baek AU - Zhaoqiang Wang AU - Susana Cavallero AU - Sandro Satta AU - Angela Lai AU - Ryan O’Donnell AU - Vijay Vedula AU - Yichen Ding AU - Alison Lesley Marsden AU - Tzung Hsiai Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/04/06/2021.02.10.430654.abstract N2 - Wall shear stress (WSS) in the micro-vasculature contributes to biomechanical cues that regulate mechanotransduction underlying vascular development, regeneration, and homeostasis. We hereby elucidate the interplay between hemodynamic shear forces and luminal remodeling in response to vascular injury and regeneration in the zebrafish model of tail amputation. Using the transgenic Tg(fli1:eGFP; Gata1:ds-red) line, we were able to track the enhanced green-fluorescent protein (eGFP)-labeled endothelial lining of the 3-D microvasculature for post-image segmentation and reconstruction of fluid domain for computational fluid dynamics (CFD) simulation. At 1 day post amputation (dpa), dorsal aorta (DA) and posterior cardinal vein (PCV) were severed, and vasoconstriction developed in the dorsal longitudinal anastomotic vessel (DLAV) with a concomitant increase in WSS in the segmental vessels (SV) proximal to the amputation site and a decrease in WSS in SVs distal to amputation. Simultaneously, we observed angiogenesis commencing at the tips of the amputated DLAV and PCV where WSS was minimal in the absence of blood flow. At 2 dpa, vasodilation occurred in a pair of SVs proximal to amputation, resulting in increased flow rate and WSS, whereas in the SVs distal to amputation, WSS normalized to the baseline. At 3 dpa, the flow rate in the arterial SV proximal to amputation continued to rise and merged with DLAV that formed a new loop with PCV. Thus, our CFD modeling uncovered a well-coordinated micro-vascular adaptation process following tail amputation, accompanied by the rise and fall of WSS and dynamic changes in flow rate during vascular regeneration.Competing Interest StatementThe authors have declared no competing interest. ER -