PT - JOURNAL ARTICLE AU - M Germuska AU - RC Stickland AU - AM Chiarelli AU - HL Chandler AU - RG Wise TI - Quantitative mapping of cerebral oxygen metabolism using breath-hold calibrated fMRI AID - 10.1101/2021.04.08.438939 DP - 2021 Jan 01 TA - bioRxiv PG - 2021.04.08.438939 4099 - http://biorxiv.org/content/early/2021/04/09/2021.04.08.438939.short 4100 - http://biorxiv.org/content/early/2021/04/09/2021.04.08.438939.full AB - Magnetic resonance imaging (MRI) offers the possibility to non-invasively map the rate of cerebral metabolic oxygen consumption (CMRO2), which is essential for understanding and monitoring neural function in both health and disease. Existing methods of mapping CMRO2, based on respiratory modulation of arterial spin labelling (ASL) and blood oxygen level dependent (BOLD) signals, require lengthy acquisitions and independent modulation of both arterial oxygen and carbon dioxide levels. Here, we present a new simplified method for mapping the rate of cerebral oxygen metabolism that can be performed using a simple breath-holding paradigm. The method incorporates flow-diffusion modelling of oxygen transport and physiological constraints to create a non-linear mapping between the maximum BOLD signal, M, baseline blood flow (CBF0), and CMRO2. A gradient boosted decision tree is used to learn this mapping directly from simulated MRI data. Modelling studies demonstrate that the proposed method is robust to variation in cerebral physiology and metabolism. This new gas-free methodology offers a rapid and pragmatic alternative to existing dual-calibrated methods, removing the need for specialist respiratory equipment and long acquisition times. In-vivo testing of the method, using an 8-minute 45 second protocol of repeated breath-holding, was performed on 15 healthy volunteers, producing quantitative maps of cerebral blood flow (CBF), oxygen extraction fraction (OEF), and CMRO2.Competing Interest StatementThe authors have declared no competing interest.