RT Journal Article
SR Electronic
T1 A gene regulatory network for neural induction
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.04.16.440164
DO 10.1101/2021.04.16.440164
A1 Katherine E. Trevers
A1 Hui-Chun Lu
A1 Youwen Yang
A1 Alexandre Thiery
A1 Anna C. Strobl
A1 Božena Pálinkášová
A1 Nidia M. M. de Oliveira
A1 Irene M. de Almeida
A1 Mohsin A. F. Khan
A1 Natalia Moncaut
A1 Nicholas M. Luscombe
A1 Leslie Dale
A1 Andrea Streit
A1 Claudio D. Stern
YR 2021
UL http://biorxiv.org/content/early/2021/04/16/2021.04.16.440164.abstract
AB During early vertebrate development, signals from a special region of the embryo, the organizer, can redirect the fate of non-neural ectoderm cells to form a complete, patterned nervous system. This is called neural induction and has generally been imagined as a single signaling event, causing a switch of fate. Here we undertake a comprehensive analysis, in very fine time-course, of the events following exposure of ectoderm to the organizer. Using transcriptomics and epigenomics we generate a Gene Regulatory Network comprising 175 transcriptional regulators and 5,614 predicted interactions between them, with fine temporal dynamics from initial exposure to the signals to expression of mature neural plate markers. Using in situ hybridization, single-cell RNA-sequencing and reporter assays we show that neural induction by a grafted organizer mimics normal neural plate development. The study is accompanied by a comprehensive resource including information about conservation of the predicted enhancers in different vertebrate systems.Competing Interest StatementThe authors have declared no competing interest.