PT  - JOURNAL ARTICLE
AU  - Clough, Mitchell
AU  - Chen, Ichun Anderson
AU  - Park, Seong-Wook
AU  - Ahrens, Allison M.
AU  - Stirman, Jeffrey N.
AU  - Smith, Spencer L.
AU  - Chen, Jerry L.
TI  - Flexible Simultaneous Mesoscale Two-Photon Imaging Of Neural Activity at High Speeds
AID  - 10.1101/2021.04.19.440507
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.04.19.440507
4099  - http://biorxiv.org/content/early/2021/04/20/2021.04.19.440507.short
4100  - http://biorxiv.org/content/early/2021/04/20/2021.04.19.440507.full
AB  - Understanding brain function requires monitoring local and global brain dynamics. Two-photon imaging of the brain across mesoscopic scales has presented trade-offs between imaging area and acquisition speed. We describe a flexible cellular resolution two-photon microscope capable of simultaneous video rate acquisition of four independently targetable brain regions spanning an approximate five-millimeter field of view. With this system, we demonstrate the ability to measure calcium activity across mouse sensorimotor cortex at behaviorally relevant timescales.Competing Interest StatementThe authors have declared no competing interest.