RT Journal Article SR Electronic T1 Flexible Simultaneous Mesoscale Two-Photon Imaging Of Neural Activity at High Speeds JF bioRxiv FD Cold Spring Harbor Laboratory SP 2021.04.19.440507 DO 10.1101/2021.04.19.440507 A1 Mitchell Clough A1 Ichun Anderson Chen A1 Seong-Wook Park A1 Allison M. Ahrens A1 Jeffrey N. Stirman A1 Spencer L. Smith A1 Jerry L. Chen YR 2021 UL http://biorxiv.org/content/early/2021/04/20/2021.04.19.440507.abstract AB Understanding brain function requires monitoring local and global brain dynamics. Two-photon imaging of the brain across mesoscopic scales has presented trade-offs between imaging area and acquisition speed. We describe a flexible cellular resolution two-photon microscope capable of simultaneous video rate acquisition of four independently targetable brain regions spanning an approximate five-millimeter field of view. With this system, we demonstrate the ability to measure calcium activity across mouse sensorimotor cortex at behaviorally relevant timescales.Competing Interest StatementThe authors have declared no competing interest.