PT  - JOURNAL ARTICLE
AU  - Bo-Ruei Chen
AU  - Yinan Wang
AU  - Anthony T. Tubbs
AU  - Dali Zong
AU  - Faith C. Fowler
AU  - Nicholas Zolnerowich
AU  - Wei Wu
AU  - Amelia Bennett
AU  - Chun-Chin Chen
AU  - Wendy Feng
AU  - Andre Nussenzweig
AU  - Jessica K. Tyler
AU  - Barry P. Sleckman
TI  - LIN37-DREAM Prevents DNA End Resection and Homologous Recombination at DNA Double Strand Breaks in Quiescent Cells
AID  - 10.1101/2021.04.21.440786
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.04.21.440786
4099  - http://biorxiv.org/content/early/2021/04/21/2021.04.21.440786.short
4100  - http://biorxiv.org/content/early/2021/04/21/2021.04.21.440786.full
AB  - DNA double strand break (DSB) repair by homologous recombination (HR) is thought to be restricted to the S- and G2- phases of the cell cycle in part due to 53BP1 antagonizing DNA end resection in G1-phase and non-cycling quiescent (G0) cells. Here, we show that LIN37, a component of the DREAM transcriptional repressor, functions in a 53BP1-independent manner to prevent DNA end resection and HR in G0 cells. Loss of LIN37 leads to expression of HR proteins, including BRCA1, BRCA2, PALB2 and RAD51, and DNA end resection in G0 cells even in the presence of 53BP1. In contrast to 53BP1-deficiency, DNA end resection in LIN37-deficient G0 cells depends on BRCA1 and leads to RAD51 filament formation and HR. LIN37 is not required to protect DNA ends in cycling cells at G1-phase. Thus, LIN37 regulates a novel 53BP1-independent cell phase-specific DNA end protection pathway that functions uniquely in quiescent cells.Competing Interest StatementThe authors have declared no competing interest.