RT Journal Article
SR Electronic
T1 Highly parallel direct RNA sequencing on an array of nanopores
JF bioRxiv
FD Cold Spring Harbor Laboratory Press
DO 10.1101/068809
A1 Garalde, Daniel R
A1 Snell, Elizabeth A
A1 Jachimowicz, Daniel
A1 Heron, Andrew J
A1 Bruce, Mark
A1 Lloyd, Joseph
A1 Warland, Anthony
A1 Pantic, Nadia
A1 Admassu, Tigist
A1 Ciccone, Jonah
A1 Serra, Sabrina
A1 Keenan, Jemma
A1 Martin, Samuel
A1 McNeill, Luke
A1 Wallace, Jayne
A1 Jayasinghe, Lakmal
A1 Wright, Chris
A1 Blasco, Javier
A1 Sipos, Botond
A1 Young, Stephen
A1 Juul, Sissel
A1 Clarke, James
A1 Turner, Daniel J
YR 2016
UL http://biorxiv.org/content/early/2016/08/12/068809.abstract
AB Ribonucleic acid sequencing can allow us to monitor the RNAs present in a sample. This enables us to detect the presence and nucleotide sequence of viruses, or to build a picture of how active transcriptional processes are changing -- information that is useful for understanding the status and function of a sample. Nanopore-based sequencing technology is capable of electronically analysing a sample's DNA directly, and in real-time. In this manuscript we demonstrate the ability of an array of nanopores to sequence RNA directly, and we apply it to a range of biological situations. Nanopore technology is the only available sequencing technology which can sequence RNA directly, rather than depending on reverse transcription and PCR. There are several potential advantages of this approach over other RNA-seq strategies, including the absence of amplification and reverse transcription biases, the ability to detect nucleotide analogues and the ability to generate full-length, strand-specific RNA sequences. This will improve the ease and speed of RNA analysis, while yielding richer biological information.