RT Journal Article SR Electronic T1 Highly parallel direct RNA sequencing on an array of nanopores JF bioRxiv FD Cold Spring Harbor Laboratory SP 068809 DO 10.1101/068809 A1 Garalde, Daniel R. A1 Snell, Elizabeth A. A1 Jachimowicz, Daniel A1 Heron, Andrew J. A1 Bruce, Mark A1 Lloyd, Joseph A1 Warland, Anthony A1 Pantic, Nadia A1 Admassu, Tigist A1 Ciccone, Jonah A1 Serra, Sabrina A1 Keenan, Jemma A1 Martin, Samuel A1 McNeill, Luke A1 Wallace, Jayne A1 Jayasinghe, Lakmal A1 Wright, Chris A1 Blasco, Javier A1 Sipos, Botond A1 Young, Stephen A1 Juul, Sissel A1 Clarke, James A1 Turner, Daniel J YR 2016 UL http://biorxiv.org/content/early/2016/08/12/068809.abstract AB Ribonucleic acid sequencing can allow us to monitor the RNAs present in a sample. This enables us to detect the presence and nucleotide sequence of viruses, or to build a picture of how active transcriptional processes are changing – information that is useful for understanding the status and function of a sample. Oxford Nanopore Technologies’ sequencing technology is capable of electronically analysing a sample’s DNA directly, and in real-time. In this manuscript we demonstrate the ability of an array of nanopores to sequence RNA directly, and we apply it to a range of biological situations. Nanopore technology is the only available sequencing technology that can sequence RNA directly, rather than depending on reverse transcription and PCR. There are several potential advantages of this approach over other RNA-seq strategies, including the absence of amplification and reverse transcription biases, the ability to detect nucleotide analogues and the ability to generate full-length, strand-specific RNA sequences. Direct RNA sequencing is a completely new way of analysing the sequence of RNA samples and it will improve the ease and speed of RNA analysis, while yielding richer biological information.