TY - JOUR T1 - Nonlinear control of transcription through enhancer-promoter interactions JF - bioRxiv DO - 10.1101/2021.04.22.440891 SP - 2021.04.22.440891 AU - Jessica Zuin AU - Gregory Roth AU - Yinxiu Zhan AU - Julie Cramard AU - Josef Redolfi AU - Ewa Piskadlo AU - Pia Mach AU - Mariya Kryzhanovska AU - Gergely Tihanyi AU - Hubertus Kohler AU - Peter Meister AU - Sebastien Smallwood AU - Luca Giorgetti Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/04/22/2021.04.22.440891.abstract N2 - Chromosome structure in mammals is thought to regulate transcription by modulating the three-dimensional interactions between enhancers and promoters, notably through CTCF-mediated interactions and topologically associating domains (TADs)1–4. However, how chromosome interactions are actually translated into transcriptional outputs remains unclear. To address this question we use a novel assay to position an enhancer at a large number of densely spaced chromosomal locations relative to a fixed promoter, and measure promoter output and interactions within a genomic region with minimal regulatory and structural complexity. Quantitative analysis of hundreds of cell lines reveal that the transcriptional effect of an enhancer depends on its contact probabilities with the promoter through a non-linear relationship. Mathematical modeling and validation against experimental data further provide evidence that nonlinearity arises from transient enhancer-promoter interactions being memorized into longer-lived promoter states in individual cells, thus uncoupling the temporal dynamics of interactions from those of transcription. This uncovers a potential mechanism for how enhancers control transcription across large genomic distances despite rarely meeting their target promoters, and for how TAD boundaries can block distal enhancers. We finally show that enhancer strength additionally determines not only absolute transcription levels, but also the sensitivity of a promoter to CTCF-mediated functional insulation. Our unbiased, systematic and quantitative measurements establish general principles for the context-dependent role of chromosome structure in long-range transcriptional regulation.Competing Interest StatementThe authors have declared no competing interest. ER -