PT  - JOURNAL ARTICLE
AU  - Hongwei Jing
AU  - David A. Korasick
AU  - Ryan J. Emenecker
AU  - Nicholas Morffy
AU  - Edward G. Wilkinson
AU  - Samantha K. Powers
AU  - Lucia C. Strader
TI  - The F-box protein AFF1 regulates ARF protein accumulation to regulate auxin response
AID  - 10.1101/2021.04.25.441346
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.04.25.441346
4099  - http://biorxiv.org/content/early/2021/04/26/2021.04.25.441346.short
4100  - http://biorxiv.org/content/early/2021/04/26/2021.04.25.441346.full
AB  - Auxin critically regulates nearly every aspect of plant growth and development. Auxin-driven transcriptional responses are mediated through the AUXIN RESPONSE FACTOR (ARF) family of transcription factors. Although ARF protein stability is regulated via the 26S proteasome, molecular mechanisms underlying ARF stability and turnover are unknown. Here, we report the identification and functional characterization of an F-box E3 ubiquitin ligase, which we have named AUXIN RESPONSE FACTOR F-BOX1 (AFF1). AFF1 directly interacts with ARF19 and regulates its accumulation. Mutants defective in AFF1 display ARF19 protein hyperaccumulation, attenuated auxin responsiveness, and developmental defects. Together, our data suggest a new mechanism, namely control of ARF protein stability, in regulating auxin response.Competing Interest StatementThe authors have declared no competing interest.