PT  - JOURNAL ARTICLE
AU  - Dorottya A. Simon
AU  - András Tálas
AU  - Péter I. Kulcsár
AU  - Ervin Welker
TI  - PEAR: a flexible fluorescent reporter for the identification and enrichment of successfully prime edited cells
AID  - 10.1101/2021.04.26.441486
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.04.26.441486
4099  - http://biorxiv.org/content/early/2021/04/27/2021.04.26.441486.short
4100  - http://biorxiv.org/content/early/2021/04/27/2021.04.26.441486.full
AB  - Prime editing is a recently developed gene engineering tool that allows the introduction of short insertions, deletions or substitutions into the genome. However, the efficiency of prime editing, generally reaching around 10-30% editing, has not resembled its versatility. Here, Prime Editor Activity Reporter (PEAR), a sensitive fluorescent tool is introduced for the identification of single cells with prime editing activity. Possessing no background fluorescence, PEAR specifically reports on prime editing events in individual cells. By design, it ensures unrestricted flexibility for sequence variations in the full length of the target sequence. The application of PEAR as an enrichment marker of prime editing can increase the edited population by up to 70% and alleviate the burden of the otherwise time and labour consuming process of cloning of the correctly edited cells, therefore considerably improving the applicability of prime editing in fundamental research and biotechnological uses.Competing Interest StatementThe authors have declared no competing interest.