TY - JOUR T1 - Tissue-specific metabolic reprogramming during wound induced <em>de novo</em> organ formation in tomato hypocotyl explants JF - bioRxiv DO - 10.1101/2021.04.29.441912 SP - 2021.04.29.441912 AU - Eduardo Larriba AU - Ana Belén Sánchez García AU - Cristina Martínez-Andújar AU - Alfonso Albacete AU - José Manuel Pérez-Pérez Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/04/29/2021.04.29.441912.abstract N2 - Plants have remarkable regenerative capacity, which allows them to survive tissue damaging after biotic and abiotic stress. Some of the key transcription factors and the hormone crosstalk involved in wound-induced organ regeneration have been extensively studied in the model plant Arabidopsis thaliana. However, little is known about the role of metabolism in wound-induced organ regeneration.Here, we performed detailed transcriptome analysis and targeted metabolomics approach during de novo organ formation in tomato hypocotyl explants and found tissue-specific metabolic differences and divergent developmental pathways after wounding.Our results indicate that callus growth in the apical region of the hypocotyl depends on a specific metabolic switch involving the upregulation of the photorespiratory pathway and the differential regulation of photosynthesis-related genes and of the gluconeogenesis pathway.The endogenous pattern of ROS accumulation in the apical and basal region of the hypocotyl during the time-course were dynamically regulated, and contributed to tissue-specific wound-induced regeneration.Our findings provide a useful resource for further investigation on the molecular mechanisms involved in wound-induced organ formation in a crop species such as tomato.One-sentence Summary Metabolic switch during wound-induced regenerationCompeting Interest StatementThe authors have declared no competing interest. ER -