PT  - JOURNAL ARTICLE
AU  - Bayantes Dagvadorj
AU  - Peter S. Solomon
TI  - Simple and efficient heterologous expression of necrosis-inducing effectors using the model plant <em>Nicotiana benthamiana</em>
AID  - 10.1101/2021.05.02.442377
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.05.02.442377
4099  - http://biorxiv.org/content/early/2021/05/03/2021.05.02.442377.short
4100  - http://biorxiv.org/content/early/2021/05/03/2021.05.02.442377.full
AB  - Plant fungal pathogens cause devastating diseases on cereal plants and threaten global food security. During infection, these pathogens secrete proteinaceous effectors that promote disease. Some of these effectors from necrotrophic plant pathogens induce a cell death response (necrosis), which facilitates pathogen growth in planta. Characterisation of these effectors typically requires heterologous expression and microbial expression systems such as bacteria and yeast are the predominantly used. However, microbial expression systems often require optimization for any given effector and are, in general, not suitable for effectors involving cysteine bridges and posttranslational modifications for activity. Here, we describe a simple and efficient method for expressing such effectors in the model plant Nicotiana benthamiana. Briefly, an effector protein is transiently expressed and secreted into the apoplast of N. benthamiana by Agrobacterium-mediated infiltration. Two-to-three days subsequent to agroinfiltration, the apoplast from the infiltrated leaves is extracted and can be directly used for phenotyping on host plants. The efficacy of this approach was demonstrated by expressing the ToxA, Tox3 and Tox1 necrosis-inducing effectors from Parastagonospora nodorum. All three effectors produced in N. benthamiana were capable of inducing necrosis in wheat lines, and two of three showed visible bands on Coomassie-stained gel. These data suggest that N. benthamiana-agroinfiltration system is a feasible tool to obtain fungal effectors, especially those that require disulfide bonds and posttranslational modifications. Furthermore, due to the low number of proteins typically observed in the apoplast (compared to intracellular), this simple and high-throughput approach circumvents the requirement to lyse cells and further purify the target proteins that is required in other heterologous systems. Because of its simplicity and potential for high-throughput, this method is highly amenable to the phenotyping of candidate protein effectors on host plants.Competing Interest StatementThe authors have declared no competing interest.AWFApoplast washing fluidNEsNecrotrophic effectorsNtPR1spNicotiana tabacum pathogenesis-related protein 1 signal peptideSCRsmall cysteine-rich