PT - JOURNAL ARTICLE AU - Shuang Song AU - Hanzi He AU - Kerstin Gühl AU - Marieke van Bolderen-Veldkamp AU - Gonda Buijs AU - Leo A.J. Willems AU - Leónie Bentsink TI - <em>DELAY OF GERMINATION 6</em>, encoding the <em>ANAC060</em> transcription factor, inhibits seed dormancy AID - 10.1101/2021.05.03.442418 DP - 2021 Jan 01 TA - bioRxiv PG - 2021.05.03.442418 4099 - http://biorxiv.org/content/early/2021/05/03/2021.05.03.442418.short 4100 - http://biorxiv.org/content/early/2021/05/03/2021.05.03.442418.full AB - The timing of seed germination is regulated by seed dormancy. There is ample natural variation for seed dormancy among as well as within plant species. In Arabidopsis several DELAY OF GERMINATION quantitative trait loci have been identified, of which DOG1 is best studied. Here we report the identification of DOG6, a quantitative trait locus with a similar strong effect on seed dormancy as DOG1. DOG6 affects the timing of germination both in laboratory as well as in field conditions. Complementation cloning revealed that DOG6 encodes the membrane bound transcription factor ANAC060. The absence of the ANAC060 protein or its sequestration outside the nucleus results in increased seed dormancy levels. The different natural variants of ANAC060 differ for the presence of the membrane binding domain, either due to the fact that this domain is absent in the genomic sequence or because the cDNA is alternatively spliced. Our data indicates that ANAC060 regulates seed dormancy by among others binding to and regulating the expression of protein phosphatases 2C class A proteins including PROTEIN PHOSPHATASE 2CA (PP2CA), ABI FIVE BINDING PROTEIN 3 (AFP3) and HIGHLY ABA-INDUCED PP2C GENE 3 (HAI3).Significance Statement ANAC transcription factors are known to effect plant development as well as the response of plants to their environment. Here, we present the identification of DELAY OF GERMINATION 6 (DOG6), a seed dormancy quantitative trait locus that encodes the ANAC060 transcription factor. We have identified different natural alleles of ANAC060 and show that these genetic variants determine the localization of the protein. ANAC060 alleles that lack the membrane binding domain end up in the nucleus. Hence they affect transcription and as such attenuate seed dormancy.Competing Interest StatementThe authors have declared no competing interest.