RT Journal Article
SR Electronic
T1 Ribosome stalling caused by the Argonaute-microRNA-SGS3 complex regulates the production of secondary siRNAs in plants
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.09.10.288902
DO 10.1101/2020.09.10.288902
A1 Hiro-oki Iwakawa
A1 Andy Y.W. Lam
A1 Akira Mine
A1 Tomoya Fujita
A1 Kaori Kiyokawa
A1 Manabu Yoshikawa
A1 Atsushi Takeda
A1 Shintaro Iwasaki
A1 Yukihide Tomari
YR 2021
UL http://biorxiv.org/content/early/2021/05/05/2020.09.10.288902.abstract
AB The path of ribosomes on mRNAs can be impeded by various obstacles. One such example is halting of ribosome movement by microRNAs, though the exact mechanism and physiological role remain unclear. Here, we find that ribosome stalling caused by the Argonaute-microRNA-SGS3 complex regulates the production of secondary small interfering RNAs (siRNAs) in plants. We show that the double-stranded RNA-binding protein SGS3 directly interacts with the 3′ end of the microRNA in an Argonaute protein, resulting in ribosome stalling. Importantly, microRNA-mediated ribosome stalling positively correlates with efficient production of secondary siRNAs from target mRNAs. Our results illustrate a role for paused ribosomes in regulation of small RNA function that may have broad biological implications across the plant kingdom.Competing Interest StatementThe authors have declared no competing interest.