PT  - JOURNAL ARTICLE
AU  - Ryota Shimada
AU  - Emily N. Alden
AU  - Kendall Hoff
AU  - Xun Ding
AU  - Jiayi Sun
AU  - Adam M. Halasz
AU  - Wei Zhou
AU  - Jeremy S. Edwards
TI  - SARS-CoV-2 Variant Identification Using a Genome Tiling Array and Genotyping Probes
AID  - 10.1101/2021.05.11.443659
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.05.11.443659
4099  - http://biorxiv.org/content/early/2021/05/11/2021.05.11.443659.short
4100  - http://biorxiv.org/content/early/2021/05/11/2021.05.11.443659.full
AB  - With over three million deaths worldwide attributed to the respiratory disease COVID-19 caused by the novel coronavirus SARS-CoV-2, it is essential that continued efforts be made to track the evolution and spread of the virus globally. We previously presented a rapid and cost-effective method to sequence the entire SARS-CoV-2 genome with 95% coverage and 99.9% accuracy. This method is advantageous for identifying and tracking variants in the SARS-CoV-2 genome when compared to traditional short read sequencing methods which can be time consuming and costly. Herein we present the addition of genotyping probes to our DNA chip which target known SARS-CoV-2 variants. The incorporation of the genotyping probe sets along with the advent of a moving average filter have improved our sequencing coverage and accuracy of the SARS-CoV-2 genome.Competing Interest StatementThe authors declare the following competing financial interest(s): The Centrillion affiliated authors are employees and the company may commercialize the work described herein.