PT - JOURNAL ARTICLE AU - Sabrina Simon AU - Thijmen Breeschoten AU - Hans J. Jansen AU - Ron P. Dirks AU - M. Eric Schranz AU - Vera I.D. Ros TI - Genome and transcriptome analysis of the beet armyworm <em>Spodoptera exigua</em> reveals targets for pest control AID - 10.1101/2021.05.14.444148 DP - 2021 Jan 01 TA - bioRxiv PG - 2021.05.14.444148 4099 - http://biorxiv.org/content/early/2021/05/16/2021.05.14.444148.short 4100 - http://biorxiv.org/content/early/2021/05/16/2021.05.14.444148.full AB - Background The genus Spodoptera (Lepidoptera: Noctuidae) includes some of the most infamous insect pests of cultivated plants including Spodoptera frugiperda, Spodoptera litura and Spodoptera exigua. To effectively develop targeted pest control strategies for diverse Spodoptera species, genomic resources are highly desired. To this aim, we provide the genome assembly and developmental transcriptome comprising all major life stages of S. exigua, the beet armyworm. Spodoptera exigua is a polyphagous herbivore that can feed from &gt; 130 host plants including several economically important crops.Results The 419 Mb beet armyworm genome was sequenced from a female S. exigua pupa. Using a hybrid genome sequencing approach (Nanopore long read data and Illumina short read), a high-quality genome assembly was achieved (N50=1.1 Mb). An official gene set (OGS, 18,477 transcripts) was generated by automatic annotation and by using transcriptomic RNA-seq data sets of 18 S. exigua samples as supporting evidence. In-depth analyses of developmental stage-specific expression in combination with gene tree analyses of identified homologous genes across Lepidoptera genomes revealed potential Spodoptera-specific genes of interest such as mg7 and REPAT46 upregulated during 1st and 3rd instar larval stages for targeted pest-outbreak management.Conclusions The beet armyworm genome sequence and developmental transcriptome covering all major developmental stages provides critical insights into the biology of this devastating polyphagous insect pest species with a worldwide distribution. In addition, comparative genomic analyses across Lepidoptera significantly advance our knowledge to further control other invasive Spodoptera species and reveals potential lineage-specific target genes for pest control strategies.Competing Interest StatementThe authors have declared no competing interest.