RT Journal Article
SR Electronic
T1 High-throughput Raman-activated cell sorting in the fingerprint region
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.05.16.444384
DO 10.1101/2021.05.16.444384
A1 Lindley, Matthew
A1 de Pablo, Julia Gala
A1 Peterson, Jorgen Walker
A1 Isozaki, Akihiro
A1 Hiramatsu, Kotaro
A1 Goda, Keisuke
YR 2021
UL http://biorxiv.org/content/early/2021/05/17/2021.05.16.444384.abstract
AB Cell sorting is the workhorse of biological research and medicine. Cell sorters are commonly used to sort heterogeneous cell populations based on their intrinsic features. Raman-activated cell sorting (RACS) has recently received considerable interest by virtue of its ability to discriminate cells by their intracellular chemical content, in a label-free manner. However, broad deployment of RACS beyond lab-based demonstrations is hindered by a fundamental trade-off between throughput and measurement bandwidth (i.e., cellular information content). Here we overcome this trade-off and demonstrate broadband RACS in the fingerprint region (300 − 1,600 cm−1) with a record high throughput of ~50 cells per second. This represents a 100× throughput increase compared to previous demonstrations of broadband fingerprint-region RACS. To show the utility of our RACS, we demonstrate real-time label-free sorting of microalgal cells based on their accumulation of carotenoids and polysaccharide granules. These results hold promise for medical, biofuel, and bioplastic applications.Competing Interest StatementK.G. is an inventor on a pending patent related to this work filed by the Japan Patent Office (nos. PCT/JP2016/089069 and WO2017119389A1, filed on 8 January 2016). K.G. is a shareholder of CYBO and Cupido. The authors declare that they have no other competing interests.