RT Journal Article SR Electronic T1 The microbiome of Lotus nodules varies with plant health in a species-specific manner JF bioRxiv FD Cold Spring Harbor Laboratory SP 2021.05.19.441130 DO 10.1101/2021.05.19.441130 A1 Duncan B. Crosbie A1 Maryam Mahmoudi A1 Viviane Radl A1 Andreas Brachmann A1 Michael Schloter A1 Eric Kemen A1 Macarena Marín YR 2021 UL http://biorxiv.org/content/early/2021/05/19/2021.05.19.441130.abstract AB Nitrogen fixation is carried out inside nodules of legumes by symbiotic rhizobia. Rhizobia dominate the nodule microbiome, however other non-rhizobial bacteria also colonise root nodules. It is not clear whether these less abundant nodule colonisers impact nodule function. In order to investigate the relationship between the nodule microbiome and nodule function as influenced by the soil microbiome, we used a metabarcoding approach to characterise the communities inside Lotus burttii, Lotus japonicus and Lotus corniculatus nodules from plants that were either starved or healthy resulting from inoculations with different soil suspensions in a closed pot experiment. We found that the nodule microbiome of all tested Lotus species differed according to inoculum, but only that of L. burttii varied with plant health. Using a machine learning algorithm, we also found that among the many non-rhizobial bacteria inside the nodule, amplicon sequence variants that were related to Pseudomonas were the most indicative signatures of a healthy plant nodule microbiome. These results support the hypothesis that legume nodule endophytes may play a role in the overall success of root-nodule symbiosis, albeit in a plant host specific manner.Competing Interest StatementThe authors have declared no competing interest.