RT Journal Article
SR Electronic
T1 Sylites: Multipurpose markers for the visualization of inhibitory synapses
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.05.20.444651
DO 10.1101/2021.05.20.444651
A1 Vladimir Khayenko
A1 Clemens Schulte
A1 Sara L. Reis
A1 Orly Avraham
A1 Cataldo Schietroma
A1 Rafael Worschech
A1 Noah F. Nordblom
A1 Sonja Kachler
A1 Carmen Villmann
A1 Katrin G. Heinze
A1 Andreas Schlosser
A1 Ora Schueler-Furman
A1 Philip Tovote
A1 Christian G. Specht
A1 Hans Michael Maric
YR 2021
UL http://biorxiv.org/content/early/2021/05/21/2021.05.20.444651.abstract
AB We introduce Sylites – small and versatile fluorogenic affinity probes for high-contrast visualization of inhibitory synapses. Having stoichiometric labeling and exceptional selectivity for neuronal gephyrin, a hallmark protein of the inhibitory post-synapse, Sylites enable superior synapse staining compared with antibodies. Combined with super-resolution microscopy, Sylites allow precise nanoscopic measurements of the synapse. In brain tissue, Sylites reveal the three-dimensional distribution of inhibitory synapses within just an hour.Competing Interest StatementH.M.M. and V.K. filed a utility model concerning Sylites.