PT  - JOURNAL ARTICLE
AU  - Honglin Luo
AU  - Yongde Zhang
AU  - Changmian Ji
AU  - Yongzhen Zhao
AU  - Jinxia Peng
AU  - Yuhui Xu
AU  - Xiuli Chen
AU  - Yin Huang
AU  - Qingyun Liu
AU  - Pingping He
AU  - Pengfei Feng
AU  - Chunling Yang
AU  - Pinyuan Wei
AU  - Haiyan Yu
AU  - Hongkun Zheng
AU  - Yong Lin
AU  - Xiaohan Chen
TI  - Golden pompano genome resource enables discovery of valuable gene determining growth traits
AID  - 10.1101/2021.05.20.445047
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.05.20.445047
4099  - http://biorxiv.org/content/early/2021/05/22/2021.05.20.445047.short
4100  - http://biorxiv.org/content/early/2021/05/22/2021.05.20.445047.full
AB  - One important goal of fish genetic breeding is to identify valuable loci and genes that can facilitate growth and thereby productivity. Few such loci or genes have been identified in golden pompano (Trachinotus ovatus), a species of significant economic value. In this study, we produced a high-quality chromosome-level genome assembly of the golden pompano by de novo sequencing and assemblies for 2 parents and 200 F1 offspring by genome re-sequencing. We exploited these assemblies to identify loci and genes by QTL mapping, Kompetitive Allele Specific PCR (KASP) genotyping, and haplotype-based regional association analysis based on growth records of a 64 biparental and 147 individuals from a naturally occurring population. At a locus 291kb from BSNP21031, we identified a somatostatin receptor type 1-like (designated as gpsstr1) gene in which the BSNP1369 of the promoter region was highly associated with growth. Loss of sstr1a, the homolog of gpsstr1 in zebrafish, caused growth retardation. Sstr1a mediated growth via sstr2 and Wnt-gsk-3β signaling pathways. Our findings provide new insights into the underlying mechanisms controlling growth. Our strategy can serve as an effective way to uncover novel genomic information and facilitate improvement of fish growth.Competing Interest StatementThe authors have declared no competing interest.