%0 Journal Article %A Paulo L. Onuchic %A Anthony N. Milin %A Ibraheem Alshareedah %A Ashok A. Deniz %A Priya R. Banerjee %T Divalent cations can control a switch-like behavior in heterotypic and homotypic RNA coacervates %D 2019 %R 10.1101/453019 %J bioRxiv %P 453019 %X Liquid-liquid phase separation (LLPS) of RNA-protein complexes plays a major role in the cellular function of membraneless organelles (MLOs). MLOs are sensitive to changes in cellular conditions, such as fluctuations in cytoplasmic ion concentrations. To investigate the effect of these changes on MLOs, we studied the influence of divalent cations on the physical and chemical properties of RNA coacervates. Using a model arginine-rich peptide-RNA system, we predicted and observed that variations in signaling cations exert interaction-dependent effects on RNA LLPS. Changing the ionic environment has opposing effects on the propensity for heterotypic peptide-RNA and homotypic RNA LLPS, which results in a switch between coacervate types. Furthermore, divalent ion variations continuously tune the microenvironments and fluid properties of heterotypic and homotypic droplets. Our results may provide a generic mechanism for modulating the biochemical environment of RNA coacervates in a cellular context. %U https://www.biorxiv.org/content/biorxiv/early/2019/02/27/453019.full.pdf