RT Journal Article
SR Electronic
T1 Tools for accurate post hoc determination of marker location within whole-brain microscopy images
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.05.21.445133
DO 10.1101/2021.05.21.445133
A1 Adam L. Tyson
A1 Mateo Vélez-Fort
A1 Charly V. Rousseau
A1 Lee Cossell
A1 Chryssanthi Tsitoura
A1 Horst A. Obenhaus
A1 Federico Claudi
A1 Stephen C. Lenzi
A1 Tiago Branco
A1 Troy W. Margrie
YR 2021
UL http://biorxiv.org/content/early/2021/05/23/2021.05.21.445133.abstract
AB To interpret in vivo experiments designed to understand brain function, high-resolution whole-brain microscopy provides a means for post hoc determination of the location of implanted devices and recorded cells in three dimensional brain space that is a critical step for data interrogation. Here we have developed Python-based tools (brainreg and brainreg-segment) to accurately map, in a common coordinate space, the position of dye-labelled probe tracks and two-photon imaged cell populations expressing fluorescent protein. The precise location of probes and cells were validated using physiological recordings and human raters that indicate accuracy levels to less than 70µm. These flexible, open-source methodologies are expected to further evolve with need and to deliver the anatomical precision that is necessary for understanding the functional architecture of the brain.Competing Interest StatementThe authors have declared no competing interest.