PT  - JOURNAL ARTICLE
AU  - Yeounsun Oh
AU  - Wi-jae Lee
AU  - Hanseop Kim
AU  - Lee Wha Gwon
AU  - Young-Hyun Kim
AU  - Young-Ho Park
AU  - Chan Hyoung Kim
AU  - Kyung-Seob Lim
AU  - Bong-Seok Song
AU  - Jae-Won Huh
AU  - Sun-Uk Kim
AU  - Bong-Hyun Jun
AU  - Cheulhee Jung
AU  - Seung Hwan Lee
TI  - Expansion of the prime editing modality with Cas9 from <em>Francisella novicida</em>
AID  - 10.1101/2021.05.25.445577
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.05.25.445577
4099  - http://biorxiv.org/content/early/2021/05/25/2021.05.25.445577.short
4100  - http://biorxiv.org/content/early/2021/05/25/2021.05.25.445577.full
AB  - Prime editing can induce a desired base substitution, insertion, or deletion in a target gene using reverse transcriptase (RT) after nick formation by CRISPR nickase. In this study, we developed a technology that can be used to insert or replace external bases in the target DNA sequence by linking reverse transcriptase to the Francisella novicida Cas 9 [FnCas9(H969A)] nickase module, which is a CRISPR-Cas9 ortholog. Using FnCas9(H969A) nickase, the targeting limitation of existing Streptococcus pyogenes Cas9 nickase [SpCas9(H840A)]-based prime editing was dramatically extended, and accurate prime editing was induced specifically for the target genes.Competing Interest StatementThe authors have declared no competing interest.