RT Journal Article
SR Electronic
T1 Adaptive spectroscopic visible-light optical coherence tomography for human retinal oximetry
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.05.28.446197
DO 10.1101/2021.05.28.446197
A1 Ian Rubinoff
A1 Roman V. Kuranov
A1 Zeinab Ghassabi
A1 Yuanbo Wang
A1 Lisa Beckmann
A1 David A. Miller
A1 Behnam Tayebi
A1 Gadi Wollstein
A1 Hiroshi Ishikawa
A1 Joel S. Schuman
A1 Hao F. Zhang
YR 2021
UL http://biorxiv.org/content/early/2021/05/30/2021.05.28.446197.abstract
AB Alterations in the retinal oxygen saturation (sO2) and oxygen consumption are associated with nearly all blinding diseases. A technology that can accurately measure retinal sO2 has the potential to improve ophthalmology care significantly. Recently, visible-light optical coherence tomography (vis-OCT) showed great promise for noninvasive, depth-resolved measurement of retinal sO2 as well as ultra-high resolution anatomical imaging. We discovered that spectral contaminants (SC), if not correctly removed, could lead to incorrect vis-OCT sO2 measurements. There are two main types of SCs associated with vis-OCT systems and eye conditions, respectively. Their negative influence on sO2 accuracy is amplified in human eyes due to stringent laser power requirements, eye motions, and varying eye anatomies. We developed an adaptive spectroscopic vis-OCT (Ads-vis-OCT) method to iteratively remove both types of SCs. We validated Ads-vis-OCT in ex vivo bovine blood samples against a blood-gas analyzer. We further validated Ads-vis-OCT in 125 unique retinal vessels from 18 healthy subjects against pulse-oximeter readings, setting the stage for clinical adoption of vis-OCT.Competing Interest StatementHFZ, RVK, and YW have financial interest in Opticent Health