RT Journal Article SR Electronic T1 Skeletal Muscle Histidine Containing Dipeptide Contents are Increased in Freshwater Turtles (Chrysemys picta bellii) with Cold-Acclimation JF bioRxiv FD Cold Spring Harbor Laboratory SP 2021.05.31.446418 DO 10.1101/2021.05.31.446418 A1 Eimear Dolan A1 Daniel E. Warren A1 Roger C. Harris A1 Craig Sale A1 Bruno Gualano A1 Bryan Saunders YR 2021 UL http://biorxiv.org/content/early/2021/05/31/2021.05.31.446418.abstract AB Freshwater turtles found in higher latitudes can experience extreme challenges to acid-base homeostasis while overwintering, due to a combination of cold temperatures along with the potential for environmental hypoxia. Histidine containing dipeptides (HCDs; carnosine, anserine and balenine) may facilitate pH regulation in response to these challenges, through their role as pH buffers. We measured the HCDs content of three tissues (liver, cardiac muscle and skeletal muscle) from the anoxia-tolerant painted turtle (Chrysemys picta bellii) acclimated to either 3 or 20°C. HCDs were detected in all tissues, with the highest content shown in the skeletal muscle. Turtles acclimated to 3°C had more HCD in their skeletal muscle than those acclimated to 20°C (carnosine = 20.8±4.5 vs 12.5±5.9 mmol·kg DM-1; ES = 1.59 (95%CI: 0.16 – 3.00), P = 0.013). The higher HCD content observed in the skeletal muscle of the cold-acclimated turtles suggests a role in acid-base regulation in response to physiological challenges associated with living in the cold, with the increase possibly related to the temperature sensitivity of carnosine’s dissociation constant and buffering power of the skeletal muscle during anoxic submergence.HighlightspH regulation is a major challenge for overwintering freshwater turtles.Histidine containing dipeptides are important intracellular buffers.Turtles acclimated to 3°C had higher HCD content than those at 20°C.HCDs may be important pH regulators in cold-acclimated turtles.Competing Interest StatementThe authors have declared no competing interest.