RT Journal Article
SR Electronic
T1 Tracking-based rolling angles recovery method for holographic tomography of flowing cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.06.01.446558
DO 10.1101/2021.06.01.446558
A1 Daniele Pirone
A1 Pasquale Memmolo
A1 Francesco Merola
A1 Lisa Miccio
A1 Martina Mugnano
A1 Amedeo Capozzoli
A1 Claudio Curcio
A1 Angelo Liseno
A1 Pietro Ferraro
YR 2021
UL http://biorxiv.org/content/early/2021/06/01/2021.06.01.446558.abstract
AB Holographic Tomography (HT) is an emerging label-free technique for microscopic bioimaging applications, that allows reconstructing the three-dimensional (3D) refractive index (RI) distribution of biological specimens. Recently, an in-flow HT technique has been proposed in which multiple digital holograms are recorded at different viewing angles around the sample while it flows and rotates within a microfluidic channel. However, unlike conventional HT methods, there is no a priori information about cell 3D orientations, that are instead requested to perform any tomographic algorithm. Here we investigate a tracking-based rolling angles recovery method, showing robustness against the sample’s features. It is based on a phase images similarity metric recently demonstrated, that exploits the local contrast phase measurements to recognize a full cell rotation within the microfluidic channel. Hence, the orientations of the flowing cells are retrieved from their positions, which are in turn computed through the 3D holographic tracking. The performances of the rolling angles recovery method have been assessed both numerically, by simulating a 3D cell phantom, and experimentally, by reconstructing the 3D RI tomograms of two cancer cells. Both the numerical and the experimental analysis have been performed at different spatial resolutions. This rolling angles recovery method, not depending on the cell shapes, the RI contents, and the optical experimental conditions, could pave the way to the study of circulating tumor cells (CTCs) in the challenging tool of liquid biopsy.Competing Interest StatementThe authors have declared no competing interest.