RT Journal Article
SR Electronic
T1 GlyConnect: a glycan-based conjugation extension of the GlycoDelete technology
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.06.02.446789
DO 10.1101/2021.06.02.446789
A1 Wander Van Breedam
A1 Karel Thooft
A1 Francis Santens
A1 Sandrine Vanmarcke
A1 Elise Wyseure
A1 Bram Laukens
A1 Berre Van Moer
A1 Wim Nerinckx
A1 Simon Devos
A1 Annemieke Madder
A1 Nico Callewaert
YR 2021
UL http://biorxiv.org/content/early/2021/06/04/2021.06.02.446789.abstract
AB Recently, our lab developed GlycoDelete, a technology suite that allows a radical simplification of eukaryotic N-glycosylation. The technology allows to produce glycoproteins that carry single GlcNAc, LacNAc, or LacNAc-Sia type glycans on their N-linked glycosylation sequons. GlycoDelete-type N-glycans are uniquely suited for glycan-based conjugation purposes, as these provide a short, homogeneous and hydrophilic link to the protein backbone. Targeting GlycoDelete-glycans allows for highly site-specific conjugation at sites in the protein which are normally occupied by bulky glycans, thus ensuring minimal interference with protein structure and function. The current manuscript describes the evaluation and optimization of both chemical and chemo-enzymatic conjugation of molecules onto the GlycoDelete-type glycans of a limited set of benchmark proteins.Competing Interest StatementThe authors have declared no competing interest.