TY - JOUR T1 - Degradation of non-methylated DNA by MsPJI impairs its usefulness as epigenetic tool JF - bioRxiv DO - 10.1101/072090 SP - 072090 AU - María Belén Jerez AU - Maximiliano Juri Ayub Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/08/29/072090.abstract N2 - DNA treatment with sensitive and/or dependent restriction enzymes, followed by PCR amplification is a widely used approach for testing CpG methylation. Recently, MspJI has been characterized as a promisory tool for epigenetic analyses. In the present report, we describe that MspJI shows significant activity against non-methylated DNA, suggesting that additional caution and improvements would be required before applying this enzyme as a routine epigenetic tool. ER -