RT Journal Article
SR Electronic
T1 Joint degeneration in a mouse model of pseudoachondroplasia: ER stress, inflammation and autophagy blockage
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.06.04.447121
DO 10.1101/2021.06.04.447121
A1 Jacqueline T. Hecht
A1 Alka C. Veerisetty
A1 Mohammad G. Hossain
A1 Debabrata Patra
A1 Frankie Chiu
A1 Francoise Coustry
A1 Karen L. Posey
YR 2021
UL http://biorxiv.org/content/early/2021/06/04/2021.06.04.447121.abstract
AB Pseudoachondroplasia (PSACH), a short limb skeletal dysplasia, associated with premature joint degeneration is caused by misfolding mutations in cartilage oligomeric matrix protein (COMP). Here, we define mutant-COMP-induced stress mechanisms that occur in articular chondrocytes of MT-COMP mice, a murine model of PSACH. The accumulation of mutant-COMP in the ER occurred early in MT-COMP articular chondrocytes and stimulated inflammation (TNFα) at 4 wks. Articular chondrocyte death increased at 8 wks and ER stress through CHOP was elevated by 12 wks. Importantly, blockage of autophagy (pS6), the major mechanism which clears the ER, sustained cellular stress in MT-COMP articular chondrocytes. Degeneration of MT-COMP articular cartilage was similar to that observed in PSACH and was associated with increased MMPs, degradative enzymes. Moreover, chronic cellular stresses stimulated senescence. Senescence-associated secretory phenotype (SASP) may play a role in generating and propagating a pro-degradative environment in the MT-COMP murine joint. The loss of CHOP or resveratrol treatment from birth preserved joint health in MT-COMP mice. Taken together, these results indicate that ER stress/CHOP signaling and autophagy blockage are central to mutant-COMP joint degeneration and MT-COMP mice joint health can be preserved by decreasing articular chondrocyte stress. Future joint sparing therapeutics for PSACH may include resveratrol.Competing Interest StatementThe authors have declared no competing interest.