RT Journal Article
SR Electronic
T1 MiR-384 Regulates Reelin by Inhibiting ADAMTS4 in Neuronal Cell Lines
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.06.04.447133
DO 10.1101/2021.06.04.447133
A1 Qiushi Cao
A1 Bisheng Huang
A1 Ping Wang
A1 Gang Zhao
A1 Min Zhao
A1 Xiao-Ming Yu
YR 2021
UL http://biorxiv.org/content/early/2021/06/04/2021.06.04.447133.abstract
AB MicroRNAs (miRNAs) are important regulators of gene expression at the post-transcriptional level. The present study aims to investigate the role of miR-384 in Reelin by regulating ADAMTS4 in neuronal cell lines. Brain tissues from Aβ1-42 induced mouse model of Alzheimer’s disease and the control group were collected. RT-PCR, Western blotting and immunohistochemistry were performed to detect the levels of ADAMTS4 and miR-384 in tissues. Luciferase reporter assay, Western blotting and in vitro assay were used to validate that ADAMTS4 was the target gene of miR-384. Neuronal cell line, Neuro-2a, was selected for transfection assay. ADAMTS4 was significantly down-regulated in hippocampi of Alzheimer’s disease mouse model, and negatively correlated with miR-384. Then, ADAMTS4 was identified as a direct target of miR-384. Over-expressing of miR-384 in Neuro-2a showed that ADAMTS4 and the cleaved Reelin fragments were down regulated, and proliferation of neuronal cell lines (Neuro-2a and SH-SY5Y) were inhibited through DAB-1 pathway. In conclusion, these results revealed that miR-384 may play a regulatory role in Reelin via inhibiting ADAMTS4 in neuronal cell lines.Competing Interest StatementThe authors have declared no competing interest.