PT  - JOURNAL ARTICLE
AU  - Tongrui Hao
AU  - Nan Feng
AU  - Fan Gong
AU  - Jiaquan Liu
AU  - Lu Ma
AU  - Yu-Xuan Ren
TI  - Complexin-1 regulated transition in the assembly of single neuronal SNARE complex
AID  - 10.1101/2021.06.07.447331
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.06.07.447331
4099  - http://biorxiv.org/content/early/2021/06/07/2021.06.07.447331.short
4100  - http://biorxiv.org/content/early/2021/06/07/2021.06.07.447331.full
AB  - Neurotransmitter release is mediated by the synaptic vesicle exocytosis. Important proteins in this process have been identified including the molecular machine Synaptic-soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) proteins, and other regulators. Complexin (Cpx) is one of the vital regulators in this process. The functions of Cpx are proposed to maintain a proper primed vesicle pool by preventing its premature depletion, which facilitates the vesicle fusion in the presence of Ca2+. However, the molecular mechanism remains unclear. Using dual-trap optical tweezers, we detected the interaction of complexin-1 (CpxI) with SNARE. We found that the CpxI stabilizes partially folded SNARE complexes by competing with C-terminal of Vamp protein and interacting with the C-terminal of t-SNARE complex.Competing Interest StatementThe authors have declared no competing interest.